Livestock Studies 1995, Vol 35, Num, 1-2     (Pages: 021-029)

Daily motility of Holstein Bull Semen Diluted with Laiciphos 488 and Stored in refrigerator (+4 °C)

Eşref DEMİRCİ 1 ,Seyfettin GÜR 2 ,Hüseyin KİNET 3 ,Azmi EROĞLU 3

1 Doç. Dr., F. Ü. Vet. Fak. Reprod. ve Sun'i Tohumlama Bilim Dalı, Elazığ / TÜRKİYE
2 Arş. Gör., F.Ü. Vet. Fak. Reprod. ve Sun'i Tohumlama Bilim Dalı, Elazığ / TÜRKİYE
3 Vet. Hek., Lalahan Hay. Aras. Enstitüsü, Sun'i Toh. Lab. Lalahan-Ankara /TÜRKİYE
- This investlgatlon was made to estlmate the daily motility of bull semen that were diluted with laiciphos 488 and stored in the refrigerator (+ 4 °C).

Five Holstein bulls aged 4 - 5 and malntalned on Lalahan Zootechnical Research Enstitute were constituted the material of this study. From each bull 10 ejeculates were collected twice a week using an artificial vagina during April and May in 1994.

From the each ejeculate 0.2 ml was transferred to a tube and mixed with laiciphos 488 and made up to 5 ml. Prior to storage in refrigerator, the first motility estimation was made and recorded. Afterwards, motility estimation on the bull semen kept in refrigarator were repeated once a 24 h for 240 hours. Motility estimation of semen were made subjectivelly by observing the movement of the spermatozoa under the microscope on a warmed stage at about 35 °C.

At the hours of semen collection motility of semen between the bulls ranged from 81 ± 1.0 % 77 ± 2.1 % with an avareged of 79.6 ± 0.8 %. The averege semen motility of 5 bulls, 24 h and 48 h after storage in the refrigerator was 62.8 ± 1.7 and 51.2 ±1.7 %, respectively. After 72 hours motility of semen was below 50 % in all the bulls and decreased gradually day by day. The motility of semen of 3 bulls disappeared at the 192 hours, and that of 2 at the 240 hours. Keywords :