Livestock Studies
2025, Vol 65, Num, 1 (Pages: 001-007)
Sperm DNA Damages and Damage Detection Methods: Current Approachs
Firdevs YILMAZ DAYANÇ 1 ,Nurdan COŞKUN ÇETİN 1
1 Hatay Mustafa Kemal University, Department of Reproduction and Artificial Insemination, Hatay, Turkey
DOI :
10.46897/livestockstudies.1703717
-
There are many factors affecting male fertility, whose causes are still largely
unknown. DNA damage in spermatozoa, in particular, contributes significantly to
infertility. Maintaining DNA integrity in sperm is essential for successful fertilization
and embryo development. Sources of DNA damage in sperm include errors during
chromatin packaging, DNA breaks caused by defective apoptosis, and oxidative
stress. These DNA damages are critical for male fertility and lead to issues such as
reduced fertilization rates, poor embryo quality, and lower pregnancy rates. While
routine examination methods provide a general overview of male fertility, they are
often insufficient for a definitive diagnosis of infertility and sterility. For instance,
DNA damage has been detected in 15% of spermatozoa with normal values in
standard sperm analyses. Additionally, assessing DNA damage in sperm along with
functional parameters provides insight into fertilization ability and embryonic
development. The goal here is to emphasize the importance of examining sperm
DNA to assess male fertility and identify DNA damage and its sources. Common tests
used to detect DNA damage include Aniline Blue, Toluidine Blue, Chromomycin A3
(CMA3), Sperm Chromatin Dispersion Test (SCD), TUNEL, Single Cell Gel
Electrophoresis (COMET), Sperm Chromatin Structure Assay (SCSA), and Acridine
Keywords :
Spermatozoon DNA damage Apoptosis Reactive Oygen Species DNA damage detection