UN SDG
Livestock Studies
2025, Vol 65, Num, 1 (Pages: 040-048)
Evaluation of Bull Semen Frozen with Different Antioxidants
2 Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Burdur, Türkiye
3 Republic of Türkiye Ministry of Agriculture and Forestry, General Directorate Food and Control, Ankara, Türkiye
4 Selcuk University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Konya, Türkiye DOI : 10.46897/livestockstudies.1731736 - In this project, ejaculates (n=34) were collected from three Brown Swiss bulls using the artificial vagina method, then divided into seven equal parts and diluted to 60×10⁶ spermatozoa/mL using the following extenders: Control, Control+1mM carnosic acid (CR1), Control+3mM carnosic acid (CR3), Control+2.5mM glutamine (G2.5), Control+7.5mM glutamine (G7.5), Control+CR1+G7.5, and Control+CR3+G2.5. All diluted aliquots were frozen after 3 hours of equilibration. Thawed samples were analyzed for motility, plasma membrane and acrosome integrity (PMAI), mitochondrial membrane potential (MMP), and DNA integrity. The Control, G7.5, and G2.5 groups showed the highest total and progressive motility. The highest PMAI value was observed in the Control group, while the lowest was in the CR3 group. The PMAI values of G2.5, G7.5, and G7.5+CR1 were significantly higher than those of CR1, CR3, and CR3+G2.5. There were no statistically significant differences in MMP among the groups. The lowest DNA damage was observed in the Control group and the highest in CR3+G2.5. Control, G2.5, G7.5, and CR1+G7.5 groups had statistically similar kinematic values (VAP, VSL, VCL) and outperformed the others. Although 2.5 mM and 7.5 mM glutamine showed a synergistic interaction, they did not significantly improve spermatological parameters. Carnosic acid appeared to act as an antagonistic antioxidant. Keywords : Bull semen Carnosic acid Glutamine
