Freezing and Transfering on Bovine Embriyos
2 Doç. Dr. A. Ü. Vet. Fak. Sun'i Tohumlama ve Reprod. Ana Billm Dalı - In this study, the result of freezing of cattle embriyos and reproductivity obtained by embryo transfer in Turkey were evaluated. The material of the study consisted of Brown Swiss and Black-White 5 cows of 5 - 6 years as donors and 14 heifers of 2 - 4 years as recipients from Konya and Lalahan Animal Livestock Research Institutes.
Superovulation and synchronisation in the research was induced by intra-muscular administration of 2500 I.U. PMSG to donors on the 10 th day of estrus cycle and 25 mg dinoprost tromethamine administration to both donors and recipients 48 hours after PMSG administration. Embriyos were obtained by using Dulbecco Phosphate Buffer Saline (M-PBS) and foley catheters having two ways by recto-vaginal route. 1.5 M glicerol as frezing solution and 0.25 ml straws were used for freezing.
Transfer to recipients were carried out on the 6 - 7 th day of cycle bay recto-vaginal route. Of 5 reciepient cattle, one did not respond to super ovulation and 17 embriyos transferable for transfer out of 24 ova/embryos were frozen.
Average number of ova per donor and transferable embryos were found (24/4) 6.0 and (17/4) 4.25 respectively.
Gestation of recipients were determined by detecting plasma progesteron levels by RIA method on the 21 st of cycle and by recto-vaginal exploration on the 50 th day. According to the 21 st day RIA data from 14 embryos trasfered, gestation rate was found (14/ 6) 42.85 % and to the 50 th day rectal palpation findings it was found (14/4) 28.57 %.
The results obtained suggested that there are some factors effecting the efficiency of embryo transfer and that gestation rate can by increased by acquiring experience in transfer of fresh or frozen embryos and by implementing a program based on assessing progesteron levels in milk or plasma in superovulation or syncrhronisation treatment.
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